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Image Search Results
Journal: Cell & Bioscience
Article Title: M1-BMDMs with Wnt5a deletion attenuate liver fibrosis by suppression of Wnt5a/Frizzled 2 axis in hepatic progenitors
doi: 10.1186/s13578-025-01467-x
Figure Lengend Snippet: M1-BMDM Wnt5a −KD injection inhibits HPCs activation and DR. ( a ) CK7 and CK19 immunostaining (×200). ( b ) EpCam and Sox9 immunostaining (×200). ( c ) Protein and mRNA expression levels of CK7, CK19, EpCAM and SOX9. ( d ) OV6/CK7 immunofluorescence co-staining (×400), and the costaining cells ratio of OV6/CK7. ( e ) OV6/CK19 immunofluorescence co-staining (×400), and the costaining cells ratio of OV6/CK19. ( f ) EpCam/CK19 immunofluorescence co-staining (×400), and the costaining cells ratio of EpCam/CK19. ( g ) Sox9/CK19 immunofluorescence co-staining (×400), and the costaining cells ratio of Sox9/CK19. * P < 0.05; ** P < 0.01
Article Snippet: The following antibodies were utilized for immunostaining and immunoblot analysis: rabbit polyclonal antibody anti-CD68 (ab125212), mouse monoclonal antibody anti-alpha smooth muscle actin (α-SMA; ab5694/ab124964), rabbit polyclonal antibodies anti-cytokeratin 7 (CK7; ab181598), EpCam (ab71916) and Sox9 (ab185230) (Abcam, Cambridge, UK); mouse monoclonal antibodies anti-transforming growth factor β1 (TGF-β1; sc-130348),
Techniques: Injection, Activation Assay, Immunostaining, Expressing, Immunofluorescence, Staining
Journal: Cell & Bioscience
Article Title: M1-BMDMs with Wnt5a deletion attenuate liver fibrosis by suppression of Wnt5a/Frizzled 2 axis in hepatic progenitors
doi: 10.1186/s13578-025-01467-x
Figure Lengend Snippet: M1-BMDM Wnt5a −OE injection promotes HPCs activation and DR. ( a ) CK7 and CK19 immunostaining (×200). ( b ) EpCam and Sox9 immunostaining (×200). ( c ) Protein and mRNA expression levels of CK7, CK19, EpCam and Sox9. ( d ) Immunofluorescence costaining of OV6/CK7 (×400) and the costaining area ratio (%). ( e ) Immunofluorescence costaining of OV6/CK19 (×400) and the costaining area ratio (%). ( f ) Immunofluorescence costaining of EpCam/CK19 (×400) and the costaining area ratio (%). ( g ) Immunofluorescence costaining of Sox9/CK19 (×400) and the costaining area ratio (%). * P < 0.05; ** P < 0.01
Article Snippet: The following antibodies were utilized for immunostaining and immunoblot analysis: rabbit polyclonal antibody anti-CD68 (ab125212), mouse monoclonal antibody anti-alpha smooth muscle actin (α-SMA; ab5694/ab124964), rabbit polyclonal antibodies anti-cytokeratin 7 (CK7; ab181598), EpCam (ab71916) and Sox9 (ab185230) (Abcam, Cambridge, UK); mouse monoclonal antibodies anti-transforming growth factor β1 (TGF-β1; sc-130348),
Techniques: Injection, Activation Assay, Immunostaining, Expressing, Immunofluorescence
Journal: Cell reports
Article Title: NAD depletion mediates cytotoxicity in human neurons with autophagy deficiency
doi: 10.1016/j.celrep.2023.112372
Figure Lengend Snippet: Key resources table
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Acetylated lysine Cell Signaling Technology Cat# 9441 Actin Sigma-Aldrich Cat# MABT1333 ATG5 Nano Tools Cat# 0262-100/ATG5-7C6 53BP1 Abcam Cat# ab36823 LC3B Novus Biologicals Cat# NB100-2220 LC3B NanoTools Cat# 0231-100/LC3-5F10 Cleaved caspase-3 (Asp175) Cell Signaling Technology Cat# 9661 GAPDH Sigma-Aldrich Cat# G8795 GFP Clontech Cat# 632375 Goat anti-mouse IgG (H+L), Alexa Fluor 488 Invitrogen Cat# A-11001 Goat anti-mouse IgG (H+L), Alexa Fluor 594 Invitrogen Cat# A-11005 Goat anti-rabbit IgG (H+L), Alexa Fluor 488 Invitrogen Cat# A-11008 Goat anti-rabbit IgG (H+L), Alexa Fluor 594 Invitrogen Cat# A-11012 Donkey anti-goat IgG (H+L), Alexa Fluor 488 Invitrogen Cat# A-11055 Goat anti-mouse IgG, H&L chain specific peroxidase conjugate Calbiochem Cat# 401253 Goat anti-rabbit IgG, H&L chain specific peroxidase conjugate Calbiochem Cat# 401393 Ki-67 Cell Signaling Technology Cat# 9449 MAP2 Invitrogen Cat# PA5-17646 NANOG R&D Systems Cat# SC009 NESTIN BioLegend Cat# 656802
Techniques: Recombinant, Cell Culture, Knock-Out, Cytotoxicity Assay, CyQUANT Assay, Proliferation Assay, TUNEL Assay, Isolation, Quantitation Assay, Activity Assay, TALENs, Software